Cell and Developmental biology

pedro-carvalho
Organelle biogenesis and homeostasis

Group Structure

GROUP LEADER:
Pedro Carvalho
POSTDOCTORAL FELLOWS:
Ombretta Foresti, Montserrat Serra (started March), Gabriel E Mora (started May), Cristina Miro (started February)
PhD STUDENTS:
Alexandra Grippa, Annamaria Ruggiano, Lisa Johnsen, Victoria Rodríguez
TECHNICIAN:
Laura Buxó, Robert Oliete (until November)

Summary

The endoplasmic reticulum (ER) is the primary site for the biogenesis of membrane and secreted proteins. Folding and maturation of these proteins is an elaborate process, frequently involving a number of posttranslational modifications (like disulfide bonds, glycosilation, lipidation, etc) and/or assembly into protein complexes. Most of the cellular lipids are also synthesized at the ER.  Perturbations in any of these processes often result in ER stress, a hallmark of many diseases such as diabetes, obesity and cancer.  Our long-term goal is to understand how these multiple functions of the ER are coordinated and integrated under different physiological conditions.
A central role of ERAD in sterol homeostasis A central role of ERAD in sterol homeostasis


Research Projects

  • Mechanisms of recognition and degradation of misfolded proteins by ERAD
  • Identification of novel regulated ERAD substrates
  • Regulation of sterol homeostasis by ERAD
  • Novel mechanisms of protein quality control in the ER
  • Mechanisms of lipid droplet biogenesis at the ER
  • Mechanisms of protein targeting to lipid droplets

Selected Publications

  1. Foresti O, Ruggiano A, Hannibal-Bach HK, Ejsing CS, Carvalho P
    “Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4.”
    eLife, 2(2):e00953 (2013) 10.7554/eLife.00953